Xenbase Image ID: 80473

FIG. 9. Overexpression of Xath5 and NeuroD promotes ectopic activation of Brn3.0 expression. Embryos were injected at the two-cell stage with RNA for ﰁ-galactosidase either alone (A) or in combination with RNA for neuroD (B) or Xath5 (C) and then cultured until stage 14 (neural plate stage). The embryos were stained with Magenta-gal (magenta color) to detect ﰁ-galactosidase expression, followed by whole-mount in situ hybridization using a digoxigenin-labeled Brn-3.0 probe (dark purple). All embryos are shown in a dorsoanterior view with the injected side on the right. (A) A control embryo injected with RNA for ﰁ-galactosidase, showing Brn-3.0 expression as two discrete patches in the anterolateral neural plate. (B) An embryo injected with RNA for neuroD and ﰁ-galactosidase showing an expanded patch of expression of Brn-3.0 in the anterolateral neural plate. (C) An embryo injected with RNA for Xath5 and ﰁ-galactosidase showing general activation of Brn-3.0 expression on the injected side with the most enhanced expression at the anterior of the embryo Image published in: Perron M et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pou4f1.L Brn3.0, brn3a, oct-t1, POU class IV homeobox 1, rdc-1 X. laevis Throughout NF stage 14 eye primordium neural plate Image source: Published Larger Image Printer Friendly View

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