Xenbase Image ID: 80514

Fig. 4. Xenopus orthologue of the hairy related gene (XHey-1) is responsive to Notch signaling in the developing brain and pronephros. Embryos were injected with either GR-Su(H)VP16, GR-NotchICD, or GR-Su(H)D B M and induced with dexamethasone either early (between stages 11 and 13) or late (between stages 15 and 19). Injected embryos were cultured, fixed between stages 24 and 30, and processed for in situ hybridization. A: In the developing brain, XHey-1 is responsive to Notch signaling (between stages 11 and 19) independently of when signaling is perturbed. Activation of Notch signaling increases expression of XHey-1 on the injected side of the head [dark orange arrows; a,c; also see 4B (i,j)], whereas suppression of Notch signaling decreases expression of XHey-1 on the injected side of the embryo (dark orange arrows; b,d). B,C: In the pronephros, XHey-1 is responsive to Notch signaling; however, this responsiveness is sensitive to the time of induction of the injected con- structs. These results can be compared with analysis of Lim-1 expression after perturbation of Notch signaling. Yellow and red arrows mark do- mains of the pronephric field (red tubule region, yellow duct region). Green arrows mark XHey-1 expression in the head region. Image published in: Rones MS et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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