Xenbase Image ID: 80520

Fig. 2. JNK is required for gut tube elongation, but not for digestive organ patterning. (A-C) Embryos were exposed to DMSO, SP600125 or Rockout (RO) from stage 35. Compared with the long coiled intestine in stage 46 DMSO controls (A), gut elongation is severely disrupted in embryos exposed to SP600125 (B) and RO (C). Decreased phosphoJun (pJun) levels in stage 46 gut extracts (inset, B) confirm the efficacy of JNK inhibition in the gut by SP600125. The efficacy of Rockout (∼74% reduction in Rho kinase activity) was confirmed using a Rho-kinase Assay Kit (Cyclex; not shown). (D-O) Gut-specific gene expression patterns were assessed by in situ hybridization in developing gut tubes isolated at stage 43 (D-F), 42 (G-L) or 45 (M-O). Appropriate region- and tissue-specific expression of Nkx-2.5 (D-F; mesoderm boundary between stomach and duodenum), Hhex (G-I; liver; G is shown in dorsal view), Pdx (J-L; pancreas and duodenal endoderm) and IFABP (M-O; intestinal endoderm) is evident under all conditions (n=6-17). FG, foregut; L, liver; MG, midgut; P, pancreas. Image published in: Dush MK and Nascone-Yoder NM (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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