Xenbase Image ID: 80523

Fig. 5. Morpholino knockdown of JNK1 results in loss of cell-cell adhesion. (A,B,D,E) Embryos were injected with a control morpholino oligonucleotide (Cont MO; A,B,F,G,J,K,N,O) or a morpholino (MO) targeting Xenopus JNK1 (JNK1 MO; D,E,H,I,L,M,P,Q). (C) Knockdown of JNK1 protein and JNK activity (phosphoJun) is evident in extracts from JNK MO-injected (mosaic) guts, compared with Cont MO-injected gut extracts. (B,E) MOs were co-injected with mCherry mRNA as a lineage tracer to confirm gut targeted injection (red). (F-Q) Sections of Cont or JNK MO-injected embryos reveal the localization of atypical protein kinase C and mCherry (aPKC, green; mCh, red; F-I), E-cadherin and laminin (Ecad, green; lam, red; J-M), and integrin-β1 (Int, green, to delineate cell outlines; N-Q) in the gut. Endoderm cells in stage 46 Cont MO-injected embryos (red cells in F-G) undergo normal intercalation and epithelial morphogenesis, as indicated by the single layer of columnar epithelial cells (N-O) with normal E-cadherin (J,K). By contrast, endoderm cells with MO-disrupted JNK1 function (red cells in H,I) do not radially intercalate or form a normal epithelium, as indicated by their irregular cell shapes (P,Q) and reduced levels of E-cadherin (L,M). Asterisks indicate an inner population of endoderm cells. (G,K,O,I,M,Q) Higher magnification images of boxed areas in F,J,N,H,L,P, respectively. Scale bars: 50 μm. Image published in: Dush MK and Nascone-Yoder NM (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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