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Fig. S2. Increasing concentrations of SP600125 and Rockout elicit increasingly short guts, but with distinct changes in tissue architecture. (A-R) Embryos were exposed to DMSO (A), SP600125 (C,E,G,I) or Rockout (K,M,O,Q) from stage 35 through 46, at the concentrations indicated. Transverse sections of a representative embryo exposed to DMSO (B) and each concentration of SP600125 (D,F,H,I) or Rockout (L,N,P,R) were stained to reveal the presence of E-cadherin (green; cell-cell adhesion) and laminin (red; basement membrane). Increasing levels of SP600125 elicit more severe gut elongation defects accompanied by decreasing levels of E-cadherin and a bilaminar endoderm architecture. Increasing levels of Rockout also elicit progressive defects in gut elongation, but E-cadherin levels remain unaffected, except for the appearance of foci of upregulated adhesive contacts (arrowheads). Image published in: Dush MK and Nascone-Yoder NM (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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