Xenbase Image ID: 80526

Fig. S3. SP600125-induced defects in adhesion precede apoptosis. (A-L) Embryos were exposed to DMSO, SP600125 or Rockout from stage 35-38 (12 hours; A-F) or 35-46 (48 hours; G-L), fixed, sectioned and immunohistochemically processed to reveal E-cadherin (Ecad, green), activated caspase 3 (casp, red) and DAPI-stained nuclei (blue) in the developing gut tube. Compared with DMSO and RO, SP600125 guts exhibit reduced and irregular E-cadherin as early as 12 hours after exposure to the inhibitor (B,E). Caspase-positive cells are normally found only in the stomach (arrow, J), but are evident in the core of SP600125 guts after 48 hours exposure (H,K). Caspase-positive cells are undetectable in the RO gut endoderm (I,L). Asterisks indicate the inner cells in the core of the gut. D-F and J-L are higher magnification images of the boxed regions in A-C and G-I, respectively. Scale bars: 50 μm. Image published in: Dush MK and Nascone-Yoder NM (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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