Xenbase Image ID: 80552

Fig. 2. Spatial transcription patterns and phenotypic effects observed after ectopic expression of Xvent-1B and Xvent-2B. Transcription patterns were analyzed by whole mount in situ hybridization of Xenopus embryos using Xvent-1B (A) or Xvent-2B (E) antisense RNA. Developmental stages shown for Xvent-1B are: 10.0 (A), 12.5 (B) and 23 (C); Xvent-2B: 10.5 (E), 12.0 (F), 24 (G), 29 (H) and 35 (I). Note that at early stages Xvent-1B expression is restricted more ventrally and lacks anterior expression in the eyes at later stages. Over-expression experiments were carried out by microinjection of either Xvent-1B (D) or Xvent-2B RNA (J) into the dorsal blastomeres of four-cell stage embryos which were then cultured until controls had reached stage 30. Note the complete ventralization and loss of all anterior structures after ectopic expression of Xvent-1B as well as of Xvent-2B. Image published in: Rastegar S et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ventx1.S posterior-ventral 1, PV.1, ventx1.1, ventx1.1-a, ventx1.1-b, Xvent-1b X. laevis Throughout NF stage 10 to NF stage 12.5 ventral marginal zone ventx1.S posterior-ventral 1, PV.1, ventx1.1, ventx1.1-a, ventx1.1-b, Xvent-1b X. laevis Throughout NF stage 23 circumblastoporal collar tail region posterior ventx2.S ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Sometime during NF stage 10.5 to NF stage 12 marginal zone ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone circumblastoporal collar ventx2.S ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Throughout NF stage 24 posterior periocular region tail region ventx2.S ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Throughout NF stage 29 and 30 periocular region otic vesicle pharyngeal arch dorsal fin tail bud cloaca lung ventx2.S ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. laevis Throughout NF stage 35 and 36 tail bud eye forebrain Image source: Published Larger Image Printer Friendly View

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