XB-IMG-80653
Xenbase Image ID: 80653
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Fig. 4. Role of neural plate in placodal Six1 induction. Embryos shown in lateral views (anterior to the left). Transplantations involved hosts and donors at early neural plate stage (stage 13) unless otherwise noted (see Fig. 1P for precise location of ectoderm grafted). All embryos were analysed at early tailbud stages (stage 206). (A) Grafts of anterior neural plate (ANP) in belly ectoderm strongly express Sox3 (A) and induce Eya1 (B, enlarged in insert) and Six1 (C) in surrounding ectoderm. (D) Cross-section of a neural plate graft (green GFP label) shows confinement of Six1 to host ectoderm. (G) Anterior neural plate grafts induce Six1 in surrounding belly ectoderm in 74% (28/38) when left untreated, but in only 22% (2/9) or 11% (1/9) when treated with the FGF inhibitor SU5402 or with BMP4 after transplantation, respectively. (H) Anterior neural ridge (ANR) grafts (enlarged in insert) in belly ectoderm express Six1 at their border (arrowhead) and induce it in adjacent ectoderm (arrow). Grafts of (I) posterior neural plate (PNP) or (J) stage 12 anterior neural plate do not induce Six1 in belly ectoderm. (K) ANR extirpation leads to reduction of anterior domains of placodal Six1 expression. (L) Unilateral extirpation of anterior neural plate (UNP) results in loss of placodal Six1 expression (white arrowheads; compare to Six1 expression on control side as indicated by black arrowheads) except for weak expression domain (arrow) next to remnants of neural folds (NF). (M) Six1 is expressed broadly (arrowhead) in belly ectoderm grafted unilaterally into anterior neural plate, sometimes extending into host ectoderm (arrow).
Image published in: Ahrens K and Schlosser G (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |