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Figure 2. Inducible activation of Lhx1, defines a temporal window of kidney field expansion.Embryos were injected (1xV2) with 200 pg of LL-VP16-GR mRNA at the 8-cell stage. (A–L) In situ hybridization for pax8 of embryos at stage 31, followed by MZ15 whole-mount immunostaining. (A, C, E, G, I, K) Uninjected embryos. (B, D, F, H, J, L) Injected embryos. Activation of LL-VP16-GR was controlled by addition of dexamethasone (Dex) at specified stages. Dex was added to uninjected and injected embryos at: (A, B) stage 10; (C, D) stage 12.5; (E, F) stage 15; (G, H) stage 18; (I, J) stage 21; (K, L) stage 24. (M) Bar graph with the percentage of injected embryos that showed expansion after Dex treatment at different stages. Expansion of pax8 expression was observed in 53% of the embryos for stage 10 (n = 36), 56% for stage 12.5 (n = 32), 84% for stage 15 (n = 32), 79% for stage 18 (n = 34), 40% for stage 21 (n = 45) and 24% for stage 24 (n = 21). Image published in: Cirio MC et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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