Xenbase Image ID: 80670

Fig S3. Dose-dependent expansion of the kidney field induced by LL-VP16. Embryos were injected (1xV2) with different doses of LL-VP16 mRNA at the 8-cell stage. (A, E, F, H, I, K, L) In situ hybridization of embryos at stage 20 for the early pronephric marker pax8. (D, G, J, M) Visualization of the injected side (asterisk) by the presence of fluorescein dextran. (A) Uninjected embryo. (B) Control and injected (50 pg) sides of the same embryo are shown. (E) Control and injected (100 pg) sides of the same embryo are shown. (H) Control and injected (200 pg) sides of the same embryo are shown. (K) Control and injected (300 pg) sides of the same embryo are shown. (L) Expansion of pax8 expression was observed in 93% of the embryos (n = 27). Arrow indicates the misshapen kidney field. Image published in: Cirio MC et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Gene Synonyms Species Stage(s) Tissue pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 20 intermediate mesoderm otic region Image source: Published Larger Image Printer Friendly View

Return to previous page