Xenbase Image ID: 80671

Fig S4. Analysis of secondary axis formation. Embryos were injected (1xV2) with 200 pg of LL-VP16-GR mRNA at the 8-cell stage. (A) In situ hybridization for pax8 of embryos at stage 31, followed by 12/101 whole-mount immunostaining. (A, C, E, G, I, K) Uninjected embryos. (B, D, F, H, J, L) Injected embryos. Activation of LL-VP16-GR was controlled by addition of dexamethasone (Dex) at specified stages. Dex was added to uninjected and injected embryos at: (A, B) stage 10; (C, D) stage 12.5; (E, F) stage 15; (G, H) stage 18; (I, J) stage 21; (K, L) stage 24. Image published in: Cirio MC et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Gene Synonyms Species Stage(s) Tissue pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 31 pronephric kidney Image source: Published Larger Image Printer Friendly View

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