Xenbase Image ID: 80672

Fig S5. Dose-dependent reduction of the kidney field induced by lhx1 depletion. Embryos were injected (1xV2) with different doses of lhx1-AS at the 8-cell stage. (A, E, F, H, I, K, L) In situ hybridization of embryos at stage 20 for the early pronephric marker pax8. (D, G, J, M) Visualization of the injected side (asterisk) by the presence of fluorescein dextran. (A) Uninjected embryo. (B) Control and injected (100 pg) sides of the same embryo are shown. (E) Control and injected (200 pg) sides of the same embryo are shown. (H) Control and injected (300 pg) sides of the same embryo are shown. (I) Reduction of pax8 expression was observed in 47% of the embryos and absence in 53% of the embryos (n = 34). (K) Control and injected (400 pg) sides of the same embryo are shown. Image published in: Cirio MC et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Gene Synonyms Species Stage(s) Tissue pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 20 intermediate mesoderm otic region Image source: Published Larger Image Printer Friendly View

Return to previous page