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Figure 3. Zic3 was induced by blockade of BMP4 signaling. (A) Expression of Zic3 and other marker genes in the dispersed animal cap explants were measured by RT-PCR. The dispersed caps (Dispersed) differentiated into neural cells expressing NCAM whereas not dissociated caps (Intact) differentiated into epidermal cells expressing epidermal keratin (E. keratin). Zic3 expression was detected only in the dispersed animal caps. Sibling control embryos served as a positive control (Embryo) and PCR on the same RNA without reverse transcription was done to check the absence of genomic DNA (RT−). (B) Zic3 was induced by the injection of dnBMPR mRNA. Zic3 expression was detected by whole-mount in situ hybridization of early gastrula (stage 10.25) embryos injected with control LacZ mRNA (Upper) or dnBMPR mRNA (Lower) into the ventromarginal region at two-cell stage. The embryo injected with dnBMPR mRNA showed an ectopic Zic3 expression in the ventromarginal zone (arrows in Lower) in addition to the proper site (arrowheads in Lower), which was also found in the control embryo (arrowheads in Upper) Image published in: Nakata K et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue zic3.S xzic3, Zinc finger protein of the cerebellum 3 X. laevis Throughout NF stage 10.25 upper blastopore lip marginal zone involuting marginal zone Image source: Published Larger Image Printer Friendly View

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