Xenbase Image ID: 80709

Fig. 1. The ventralizing activity of Tsg resides in its C-terminal cysteine-rich domain. (A) The Tsg mutations presented here. (B) Alignment of the N-terminal cysteine rich domain of Tsg proteins from Xenopus (xTsg), mouse (mTsg), human (hTsg), Drosophila (dTsg, dTsg2) and Chordin CR1. (C-J) Stage 42 wild-type control embryo (C) and embryos radially microinjected at the four-cell stage with a total of 2 ng xTsg mRNA (D), xTsgS36A (E), xTsgS54A (F), xTsgC59A (G), xTsgW67G (H), xTsgC198A (I), xTsgW67G+C198A (J). Each construct was analyzed in at least three independent experiments with n>30. (K) xTsg and mutant xTsg proteins secreted by dissociated animal cap cells after microinjection of 2 ng of the indicated mRNAs. Image published in: Oelgeschläger M et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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