Xenbase Image ID: 80710

Fig. 3. Wild-type xTsg and the hyperventralizing mutant xTsgW67G antagonize the dorsalization of mesoderm by Activin. (A-D) Animal cap explants were isolated at stage 8 and treated with 2 ng/ml Activin protein. Activin induces elongation (B). Microinjection of a total of 2 ng xTsg (C) or xTsgW67G (D) mRNA at the eight-cell stage into the animal blastomeres prevented elongation. (E) The accumulation of Activin-induced endogenous Chordin protein (lane 2) was reduced by wild-type xTsg mRNA injection (lane 3) and increased by xTsgW67G (lane 4). (F) RT-PCR analysis of animal cap explants treated with Activin after microinjection of 2 ng xTsg or xTsgW67G mRNA; both mRNAs inhibited the induction of dorsal mesodermal (MyoD, alpha-Actin) or neural (NCAM) marker genes by Activin. EF1α served as a loading control. Image published in: Oelgeschläger M et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page