Xenbase Image ID: 80717

Fig. 2. Time course of placodal Six1 induction. (A,B) Specification of placodal Six1 expression. (A) Six1 expression persists in most LPR explants, explanted at stage 16. (B) Percentage of LPR explants expressing Six1 after explantation at different stages and analysed after control embryos had reached early tailbud stages (stage 206). (C,D) Commitment of placodal Six1 expression. (C) Six1 expression is maintained in LPR graft (arrowheads) after isochronic transplantation into belly ectoderm at stage 15. (D) Percentage of grafts expressing Six1 after transplantation of LPR at different stages into stage 135 belly ectoderm and survival of host until early tailbud stages (stage 206). Host ectoderm was not induced to express Six1 by grafts at any stage. (E) Ectodermal competence and availability of signals for placodal Six1 induction. All host embryos were analysed after survival until early tailbud stages (stage 206). After orthotopic transplantations (E,F), stage 13 donor LPR ectoderm expresses Six1 when grafted isochronically (E), but fails to do so when grafted into stage 16 hosts (F; insert shows control side of embryo). Heterotopic transplantations (G) reveal that belly ectoderm is competent to express Six1 when grafted into the LPR of stage 13 hosts from stage 13 (G) up to at least stage 22 (I), but does not express Six1 when grafted into stage 16 hosts (H; insert shows control side of embryo). Percentage of grafts expressing Six1 after transplantation of belly ectoderm at different stages into stage 13 LPR is indicated in (J). Light blue bars indicate percentage of grafts exhibiting a disturbed pattern of Six1 expression. Image published in: Ahrens K and Schlosser G (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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