XB-IMG-80730
Xenbase Image ID: 80730
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Fig. 6. FoxB1 suppresses anterior development via the expression of Wnt and FGF ligands. (A′) Overexpression of FoxB1 results in the suppression of anterior structures such as the cement glands and eyes. GR-FoxB1 mRNA (250 pg; B and B′) were injected into four animal blastomeres of 4- to 8-cell stage embryos. Injected embryos were cultured until the tailbud stage. All panels show anterior to the left. (C) Suppression of the cement glands in embryos overexpressing FoxB1 was rescued by the inhibition of Wnt signaling. Uninjected control embryos are shown in (C). GR-FoxB1 mRNA (500 pg) was injected with (E) or without (D) Dkk mRNA (50 pg) into four animal blastomeres of 4-cell stage embryos. All panels show anterior (front) views with dorsal to the top. (F) Summary of phenotypes observed in the experiment shown in (C)E). (G) Inhibition of the Wnt pathway partially rescues defects in the expression of the retinal marker Rx2A gene caused by FoxB1 overexpression. Neurula stage embryos were injected with GR-FoxB1 mRNA (500 pg; H) alone or together with Dkk mRNA (50 pg; I) into the animal poles of 4-cell stage embryos. An uninjected control embryo is shown in (G). The expression of Rx2A gene analyzed by in situ hybridization is shown in purple. All panels show anterior (front) views with dorsal to the top. (J) Summary of phenotypes shown in (G)I). (K) FoxB1 promotes the expression of Wnt and FGF ligand genes in ectodermal explants. GR-FoxB1 mRNA (500 pg) was injected into the animal pole of 4- to 8-cell stage. Ectodermal explants were isolated at the blastula stage, treated with DEX to activate FoxB1 and cultured until gastrula (st. 12.5) or early neurula stage (st. 15/16). The relative level of expression of each gene (normalized to ODC expression) was quantified by QPCR. Image published in: Takebayashi-Suzuki K et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
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