XB-IMG-80733
Xenbase Image ID: 80733
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Fig. 3. Characterization of a novel transcript, Rbp4l, in X. laevis. A: Protein alignment showing distinct groups between retinol binding proteins and purpurin family members. B: Temporal expression of Rbp4l throughout embryonic stages of development, showing slight detection at stages 17 and 24, and an increase in expression at stages 31 and 35. Confirmation of microarray predictions by means of reverse transcriptase-polymer- ase chain reaction (RT-PCR), showing an increase in Rbp4l expression in response to Pitx3-morpholino (Pmo) at stages 19 and 27, when com- pared with wild-type (WT) and control-morpholino (Cmo) treatments. C: In situ hybridization with antisense riboprobe against Rbp4l transcript shows expression at stages 27 (C), 31 (D), and 35 (E) concentrated in the developing lens (white arrows, D and E) and at the dorsal midline of the developing midbrain region. F: An embryo injected unilaterally with Pitx3 morpholino on its right side (left of the dotted line) displayed enhanced and general expression in the craniofacial region. G: A schematic diagram of Rbp4l protein depicting a secretory signal at the N-terminus (red) and three characteristic lipocalin motifs (blue) that classify this protein as a member of the kernel subfamily of lipocalins. GenBank accession num- bers used to generate phylogenetic tree (A) are as follows: xRbp4l CD362061 (X. laevis), rRbp4 plasma BC167099 (rat), mRbp4 BC031809 (mouse), hRBP4 plasma AL356214 (human), cRbp4 precursor NM_205238 (chick), xlRbp4 precursor NM_001087726 (X. laevis), xlRb4 plasma NM_001086998 (X. laevis), xtRbp4 plasma NM_001015748 (X. tropicalis), zRbp4 NM_130920 (zebrafish), zpurpurin AB242211 (zebrafish), spurpurin NP_001135080 (salmon), ccpurpurin NP_001187969 (channel catfish), gpurpurin BAD42450 (goldfish), bcpurpurin AD028302 (blue catfish), cpur- purin P08938 (chick). Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.
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