Xenbase Image ID: 80734

Fig. 4. Characterization of a novel transcript Galectin IX in X. laevis. A: Protein alignment showing amino acid similarities between Xenopus Galectin family members. B: Temporal expres- sion of Galectin IX throughout embryonic stages of development, shows expression beginning at gastrulation (stage 10), decreasing at stage 12, and expressing consistently at stages 17 through 35. Confirmation of microarray predictions by means of reverse transcriptase-polymer- ase chain reaction (RT-PCR), detect an increase in expression at stage 19 and a decrease at stage 27 for Pitx3-morpholino (Pmo) -treated samples, compared with wild-type (WT) and con- trol-morpholino (Cmo). C: Galectin IX transcript expresses at stages 24 (C), 27 (D), and 31 (E) concentrated in the developing eye (white arrows) and presumptive pronephros, persisting in the nephric tubules and ducts. GenBank accession numbers used to generate phylogenetic tree (A) are as follows: xGalectinIa AB056478, xGalectinIb AB060969, xGalectinIIa AB060970, xGalecti- nIIb AB080016, xGalectinIIIa AB060971, xGalectinIIIb AB080017, xGalectinIVa AB060972, xGa- lectinVa M88105, xGalectinVb AB080018, xGalectinVIa AB080019, xGalectinVIIa AB080020, xGalectinVIIIa AB080021, xGalectinIX BJ056659. Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue lgals9.L ecalectin, Galectin IX, galectin-9, huat, lgals9a X. laevis Throughout NF stage 24 eye head somite optic vesicle presomitic mesoderm cranial neural crest pronephric mesenchyme lgals9.L ecalectin, Galectin IX, galectin-9, huat, lgals9a X. laevis Sometime during NF stage 27 to NF stage 31 eye head somite stomodeal-hypophyseal primordium pharyngeal arch pronephric kidney pronephric duct tail bud post-anal gut Image source: Published Larger Image Printer Friendly View

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