XB-IMG-80735
Xenbase Image ID: 80735
Fig. 5. Characterization of a novel transcript, Rdh16, in X. laevis. A: Protein alignment showing amino acid similarities between Xenopus retinol dehydrogenase (rdh) family members. B: Tem- poral expression of Rdh16 throughout embryonic stages of development shows faint expression beginning at stage 24 and 27, then increasing at stages 31 and 35. We were unable to confirm the microarray predictions by means of reverse transcriptase-polymerase chain reaction (RT- PCR), as no change in expression was detected between wild-type (WT) control-morpholino (Cmo), or Pitx3-morpholino (Pmo) embryos. C: In situ hybridization with antisense riboprobe against Rdh16 transcript, shows expression at stages 27 (C), 31 (D), and 35 (E) concentrated in the eyecup, branchial arches, and otic vesicle, as well as along the lateral plate mesoderm, with a focus on the posterior half (D), and on in the developing myotomes. GenBank accession num- bers used to generate phylogenetic tree (A) are as follows: xRdh16 NP_001083356, xRdh7 NP_001079189, xRdh13 NP_001085680, xRdh5 NP_001086194, xRdh9 NP_001090337, xRdh10 ACN32204. Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.
Image source: Published Larger Image Printer Friendly View |