Xenbase Image ID: 80736

Fig. 6. In situ hybridization analysis for putative Pitx3 target genes Obscnl and Baz2b. Visual comparisons of gene expression patterns between control-morpholino (Cmo) and Pitx3-morpho- lino (Pmo) right side-injected embryos. A: Obscnl shows a loss of expression in the branchial arches (black arrow), otic vesicle, and retina when treated at stage 27 with Pmo (A0) versus Cmo (A). B: Baz2b is substantially reduced in response to Pmo (B0) in the retinal layer of the optic protuberance (white arrow), as well as in the pronephros and in the anterior region of the dorsal axis, when compared with Cmo (B). Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue obscn.L LOC108718692, LOC108719653 X. laevis Throughout NF stage 27 otic vesicle head somite cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest eye retina hindbrain baz2b.S X. laevis Throughout NF stage 27 eye retina pronephric mesenchyme spinal cord hindbrain anterior neural tube tail bud hyoid crest mandibular crest Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.6.a^1 Expression Phenotype decreased amount obscn.L expression in otic vesicle decreased amount obscn.L expression in pharyngeal arch decreased amount obscn.L expression in retina Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.6.b^1 Expression Phenotype decreased amount baz2b.S expression in pronephric kidney decreased amount baz2b.S expression in retinal layer Larger Image Printer Friendly View

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