Xenbase Image ID: 80737

Fig. 7. Characterization of a novel transcript, Baz2b, in X. laevis. A: Protein alignment showing amino acid similarities between Baz2B homologs across organisms. B: Temporal expression of Baz2b throughout embryonic stages of development show expression as a maternal transcript in the egg E and throughout development to tailbud stage, with slight reductions in transcript level at stages 10 and 19. B Confirmation of microarray predictions by means of reverse tran- scriptase-polymerase chain reaction (RT-PCR) show abolished expression at stage 27 in response to Pitx3-morpholino (Pmo) when compared with control-morpholino (Cmo) and wild- type (WT) embryos. C: Baz2b expression at stages 21 (C), 31 (D), and 35 (E) is concentrated in the developing eye, as well as the branchial arches and otic vesicle. Dark expression is seen in the pronephros, persisting in the tubules (E). F: A schematic diagram of Baz2b protein depict- ing various domains characteristic of Baz2B: methyl-CpG binding domain (MBD), DNA binding domain (DDT), zinc finger domain (Z), adjacent to the bromodomain (BR). GenBank accession numbers used to generate phylogenetic tree (A) are as follows: xBaz2b BQ400337 (X. laevis), mBaz2b BC150814 (mouse), rBaz2b NM_001108260 (rat), hBAZ2B NM_013450 (human), cBaz2b NM_204677 (chick), xtBaz2b BC166361 (X. tropicalis). Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue baz2b.S X. laevis Throughout NF stage 21 eye primordium cranial neural crest neural tube otic placode baz2b.S X. laevis Throughout NF stage 31 otic vesicle eye pharyngeal arch mandibular arch hyoid arch branchial arch pronephric kidney spinal cord baz2b.S X. laevis Throughout NF stage 35 and 36 eye head pronephric kidney pronephric tubule Image source: Published Larger Image Printer Friendly View

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