Xenbase Image ID: 80739

Fig. 9. In situ hybridization analysis for putative segmentation targets of Pitx3. Visual comparisons of gene expression patterns between right- side injected control-morpholino (Cmo) or Pitx3-morpholino (Pmo) embryos and contralateral control. A0: Ripply2 expression, showing as two stripes in the presomitic mesoderm, shows an anterior shift (black arrow) in expression at stage 19 when treated with Pmo (A0) instead of Cmo (A). At stage 27, Ripply2 expression pattern loses its distinct shape and becomes unrestricted in response to Pmo (B0), whereas with Cmo treatment, precise patterning of this gene expression remains intact (B). C0: Hes4 expression becomes blurred in Pmo-treated embryos at stage 19 (C0) and at stage 27 (D0) Hes4 expression is absent in the presomitic mesoderm (black arrow) and pronephros areas, compared with Cmo-treated embryos (D). E0: Hes7 no longer expresses in the most anterior stripe (black arrow), and the remaining two stripes are shifted anteriorly in comparison to the contralateral control (E0). At stage 27, on the Pmo side of the embryo (F0), Hes7 shows increased expression in the presomitic mesoderm (white arrow) and again an anterior shift of the striped pattern (black arrow). Dotted line represents the midline of the embryo, separating injected right-side from contralateral left-side control. Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue ripply2.1.S ledgerline, stripy X. laevis Sometime during NF stage 19 to NF stage 27 presomitic mesoderm posterior paraxial mesoderm hes4.S H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b X. laevis Throughout NF stage 19 paraxial mesoderm cranial neural crest eye primordium hes4.S H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b X. laevis Throughout NF stage 27 brain mesoderm presomitic mesoderm eye somite pronephric kidney hes7.2.L enhancer-of-split-related 4, ESR 4, ESR-4, esr4 X. laevis Throughout NF stage 19 presomitic mesoderm posterior paraxial mesoderm hes7.2.L enhancer-of-split-related 4, ESR 4, ESR-4, esr4 X. laevis Throughout NF stage 27 mesoderm presomitic mesoderm tail region Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pitx3 MO + NF19 (in situ hybridization) fig.9.a^1 Expression Phenotype mislocalised ripply2.1.S expression in presomitic mesoderm Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.9.a^1 Expression Phenotype mislocalised ripply2.1.S expression in presomitic mesoderm Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.9.b^1 Expression Phenotype increased amount ripply2.1.S expression in presomitic mesoderm Xla Wt + pitx3 MO + NF19 (in situ hybridization) fig.9.c^1 Expression Phenotype mislocalised hes4.S expression in neural plate border increased amount hes4.S expression in neural plate border Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.9.d^1 Expression Phenotype decreased amount hes4.S expression in presomitic mesoderm decreased amount hes4.S expression in pronephric kidney Xla Wt + pitx3 MO + NF19 (in situ hybridization) fig.9.e^1 Expression Phenotype decreased amount hes7.2.L expression in paraxial mesoderm mislocalised hes7.2.L expression in paraxial mesoderm mislocalised hes7.2.L expression in presomitic mesoderm Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.9.f^1 Expression Phenotype mislocalised hes7.2.L expression in paraxial mesoderm increased amount hes7.2.L expression in presomitic mesoderm Larger Image Printer Friendly View

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