Xenbase Image ID: 80740

Fig. 10.In situ hybridization analysis for putative tailbud targets of Pitx3. A0: HoxA11 shows decreased posterior expression in the tailbud region (white arrows) of Pmo embryos at stages 19 (A0) and 27 (B0); C0 :Spr2 displays a broader and larger domain of expression (black arrows) when treated with Pmo, both at stage 19 (C0) and 27 (D0), compared with Cmo-treated embryos (C, D). E0: Lim1 expression disappears from paraxial mesoderm (red arrow) and is up-regulated in lateral mesoderm (black arrow) at stage 19 when treated with Pmo (E0). At stage 27 (F0), Pmo reduces Lim1 expression in the developing pronephros (black arrow) and in the head mesenchyme and along the dorsal axis. Image published in: Hooker L et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue hoxa11.S LOC101733863, LOC108719932, Xhoxa11 X. laevis Throughout NF stage 19 mesoderm presomitic mesoderm tail region hoxa11.S LOC101733863, LOC108719932, Xhoxa11 X. laevis Throughout NF stage 27 mesoderm presomitic mesoderm tail bud lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 19 ectoderm paraxial mesoderm lateral plate mesoderm lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 27 mesoderm presomitic mesoderm head somite pronephric kidney pronephric tubule pronephric duct sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Throughout NF stage 19 posterior paraxial mesoderm neural plate border chordal neural plate border sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Throughout NF stage 27 mesoderm tail bud Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pitx3 MO + NF19 (in situ hybridization) fig.10.a^1 Expression Phenotype decreased amount hoxa11.S expression in tail bud Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.10.b^1 Expression Phenotype decreased amount hoxa11.S expression in tail bud Xla Wt + pitx3 MO + NF19 (in situ hybridization) fig.10.c^1 Expression Phenotype increased amount lhx1.L expression in lateral plate mesoderm decreased amount lhx1.L expression in paraxial mesoderm increased amount sp5l.S expression in tail bud mislocalised sp5l.S expression in tail bud Xla Wt + pitx3 MO + NF27 (in situ hybridization) fig.10.d^1 Expression Phenotype decreased amount lhx1.L expression in head mesenchyme decreased amount lhx1.L expression in pronephric kidney increased amount sp5l.S expression in tail bud mislocalised sp5l.S expression in tail bud Larger Image Printer Friendly View

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