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XB-IMG-80782

Xenbase Image ID: 80782


FIG. 2. Expression of XNkx-2.5 during embryogenesis detected by whole-mount in situ. hybridization. The dark-stained product of the alkaline phosphatase reaction marks the position of XNkx-2.5 expression. (A) Face-on view of a cleared stage 15 embryo showing the hi-lobed expression of XNkx-2.5 (arrowed) in front of the c(osing anterior neural folds. The nonspecific dark stain on the dorsal side (up) is due to focusing through the archenteron. (B) Face-on view of an embryo that has just closed its neural folds (stage 18). Staining in the region in front of the neural folds indicates that XNkx-2.5 expression is in the presumptive heart region. (C) A cleared, side view of the embryo in (B) showing the staining at the front of the expanding archenteron (a). (D) Expression of XNk.'C-2.5 in a tailbud embryo (stage 26/27). Anterior is left and dorsal is up. Expression is concentrated in the heart, which is beginning to differentiate at this stage, but the staining extends further dorsal than the heart boundaries. (E) Ventral view of a tail bud embryo (stage 26/ 27) showing that expression in the heart region still appears hi-lobed. (F) Expression in other tissues can be detected later in development (stage 38/40). Expression continues in the heart (h), but is also visible in the anterior gut (g). The staining in the cement gland (cg) is nonspecific. (G) Ventral view of the embryo showing that the gut expression appears to be in a region that is forming a tubular structure. Anterior is up. (H) View of an embryo from above, showing thin parallel stripes of expression in the pharyngeal region (p).

Image published in: Tonissen KF et al. (1994)

Copyright © 1994. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
nkx2-5.SAR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5X. laevisThroughout NF stage 15cardiac mesoderm
nkx2-5.SAR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5X. laevisThroughout NF stage 18cardiac mesoderm
archenteron
nkx2-5.SAR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5X. laevisThroughout NF stage 26 to NF stage 27heart
cardiac mesoderm
heart primordium
nkx2-5.SAR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5X. laevisThroughout NF stage 37 and 38 to NF stage 40heart
foregut
visceral pouch

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