Xenbase Image ID: 80794

Fig. 2. Antisense TSG and chordin MOs block the in vivo activity of TSG and chordin respectively. (A) A stage matched uninjected control embryo. (B,C) Microinjection of 1 ng of Xenopus TSG mRNA per blastomere at the four-cell stage induces head and tail defects in early tailbud stage frog embryos (B), and this phenotype is inhibited by coinjecting 5 ng TSG-MO per blastomere together with the XTSG mRNA (C). (D,E) Similarly, 2.5 pg ChdA mRNA microinjected into the marginal zone of each ventral blastomere at the four-cell stage efficiently induces secondary axes (D) and these are completely blocked by ventral coinjection together with 5 ng/blastomere of ChdA-MO (E). Identical results were obtained for coinjections of ChdB-MO together with ChdB mRNA (data not shown). Coinjection of either XTSG or chordin mRNAs together with a control MO has no effect on the ability of these mRNAs to elicit these phenotypes. In all panels anterior is to the right and dorsal is to the top. 1and 2indicate primary and secondary axes. Image published in: Blitz IL et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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