Xenbase Image ID: 80802

Figure S6. Loss of ADMP Renders the Embryo Hypersensitive to Changes in BMP Levels. (A) Frontal view of uninjected neurula (stage 13) stained with telencephalic/eye marker Rx2a, hindbrain marker Krox20, and somite marker MyoD. (B) ADMP-depleted embryos display increased anterior CNS structures (marked by Rx2a) and wider axial structures (enlarged gap between paraxial MyoD expression domains). (C) BMP4-depleted embryos display moderately increased anterior CNS structures (marked by Rx2a). (D) Lowering BMP signaling levels (by injecting Chordin protein in the blastocoele cavity at stage 8) dorsalizes the embryo as shown by increased Rx2a expression, expanded Krox20 expression, and wider axial structures. (E) Lowering BMP signaling levels in ADMP-depleted embryos greatly expands dorso-anterior structures as shown by the dramatic expansion in Rx2a expression and widened axial structures. (F). Lowering BMP signaling levels with Chordin protein in BMP4-depleted embryos fails to expand telencephalic structures marked by Rx2a expression. This difference with the response to ADMP knockdown (compare to panel E) is presumably due to the compensatory up-regulation of ADMP activity on the dorsal side triggered by overall low BMP levels. Image published in: Reversade B and De Robertis EM (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 13 paraxial mesoderm egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 13 rhombomere rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 13 optic field Image source: Published Larger Image Printer Friendly View

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