Xenbase Image ID: 80849

Fig. 2. DEADSouth gene overexpression decreases the number of PGCs. (A) Representative examples of stage 32 embryos injected with mRNA for v-DS only, v-DS plus DEADSouth-DS 39UTR (DS-full) and v-DS plus nonsense DEADSouth-DS 39UTR (DS-stop), respectively. Anterior is toward the left. Scale bar: 1 mm. (A99) High magnification of the areas indicated in (A), respectively. (D) The number of PGCs per embryo at stage 32 injected with the indicated mRNAs. PGC numbers were determined by externally counting from both sides. (E) The number of PGCs per embryo at the indicated stage without/with DS-full mRNA. Embryos were dissociated, and EGFP-positive and large cells were counted as PGCs. and indicate the number of experiments and total embryos examined, respectively. The P-value was calculated by the Student t-test. Error bars indicate s.d. (F) Apoptotic PGCs in DEADSouth-overexpressing embryos at stage 28. PGCs were observed after dissociating the embryonic region (red box) immunostained for Xdazl and caspase-3. Arrowheads and arrows indicate Xdazl-positive/caspase-3- negative cells (non-apoptotic PGCs) and Xdazl positive/caspase-3-positive cells (apoptotic PGCs), respectively. Scale bar: 100 mm. Image published in: Yamaguchi T et al. (2013) Copyright © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page