Xenbase Image ID: 80850

Fig. 3. An LNA gapmer causes degradation of targeted DEADSouth mRNA. (A) Sequence alignment of DEADSouth mRNA (nucleotide position 291, accession no. AF190623), antisense and mismatch LNA gapmers and synthesized DEADSouth mRNA (DS-full). Upper letters indicate the amino acid sequence. LNA modifications and mismatch bases are underlined and in red, respectively. The sequence of DS-full mRNA was predicted from that of the template DNA. (B) Quantification of DEADSouth and Xpat mRNAs in stage 8 and 32 embryos injected with LNA gapmers. The amounts of these mRNAs were determined by real-time RT-PCR, normalized to EF1a mRNA levels as an internal control, and shown as relative amounts to the mRNA level in embryos injected with mismatch LNA gapmer. P-values and s.d. are indicated. Image published in: Yamaguchi T et al. (2013) Copyright © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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