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Fig. 4. The number of PGCs is decreased by knockdown of the DEADSouth gene. (A) Representative examples of stage 32 embryos injected with the mismatch LNA gapmer, antisense LNA gapmer, antisense LNA gapmer plus DS-full and antisense LNA gapmer plus mutant DEADSouth-DS 39UTR (DS-AAA), in addition to v-DS as a PGC-tracer, respectively. Scale bar: 1 mm. (A99) High magnification of the areas indicated in (A), respectively. (E) The number of PGCs per embryo at stage 32 injected with the indicated LNA gapmer(s) and mRNAs. PGC numbers were determined by externally counting from both sides. (F) The number of PGCs per embryo at the indicated stages with mismatch or antisense LNA gapmers. The injected mito-EGFP embryos were dissociated, and EGFP-positive and large cells were counted as PGCs. N and n indicate the number of experiments and total embryos examined, respectively. P-values were calculated by the one- tailed t test. Error bars indicate s.d. Image published in: Yamaguchi T et al. (2013) Copyright © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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