Xenbase Image ID: 80870

Fig. 6. sfrp1 inhibits wnt6 in vivo. (A) In the sfrp1 and wnt6 functional interaction assay, mRNA was injected into one ventral blastomere of 4-cell embryos. Embryos were left to develop until stages 20 or 32 for analysis. (B) Control embryo injected with nβ-gal-encoding mRNA showing normal axis development (100%). (C) Injection of nβ-gal-encoding mRNA and wnt6 mRNA into the same ventral blastomere causes secondary axis formation in over 70% of injected embryos. (D) Embryos injected with sfrp1 mRNA followed 30 minutes later by wnt6 mRNA into the same ventral blastomere show rescue of wnt6-induced secondary axis formation, being reduced to less than 20%. (E) The number of embryos injected and their phenotype. The results presented here are from at least three independent experiments (i.e. with independent batches of embryos). Image published in: Gibb N et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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