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Fig. S2. Loss-of-function experiments show no change to cardiogenic gene expression during embryonic stages 18-24. (A-F) Whole-mount RNA in situ hybridisation analysis showing nkx2-5 expression at stages 18 (A,D), 22 (B,E) and 24 (C,F) in CoMO- injected (A-C) or sfrp1MO1-injected (D-F) embryos. (G-L) Whole-mount RNA in situ hybridisation analysis showing gata6b expression at stages 18 (G,J), 22 (H,K) and 24 (I,L) in CoMO-injected (G-I) or sfrp1MO1-injected (J-L) embryos. Image published in: Gibb N et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 18 cardiac progenitor cell nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 22 heart primordium nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 24 heart primordium gata6.L GATA-6, gata6-a, gata6-b, gata6a, gata6b, tGata6, XGATA-6, XGATA6 X. laevis Throughout NF stage 24 heart primordium gata6.L GATA-6, gata6-a, gata6-b, gata6a, gata6b, tGata6, XGATA-6, XGATA6 X. laevis Throughout NF stage 22 heart primordium gata6.L GATA-6, gata6-a, gata6-b, gata6a, gata6b, tGata6, XGATA-6, XGATA6 X. laevis Throughout NF stage 18 cardiac progenitor cell Image source: Published Larger Image Printer Friendly View

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