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Fig. S2. Loss-of-function experiments show no change to cardiogenic gene expression during embryonic stages 18-24. (M,N) Quantitative analysis of nkx2-5 (M) and gata6b (N) in situ hybridisation. Note there is minimal to no loss of gene expression in embryos with loss of sfrp1 compared with controls at the indicated stages. Image published in: Gibb N et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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