Xenbase Image ID: 80878

Fig. S4. Unilateral sfrp1MO-injection experiment. (A) sfrp1MO1 was injected into the marginal zone of the dorsal blastomere on the prospective left side of the embryo. (B-E) Whole-mount RNA in situ hybridisation analysis on stage 32 embryos showing cardiac marker gene expression: nkx2-5 (B), gata6b (C), tnni3 (D) and myl2 (E). Note reduced gene expression on the left side of the embryos where sfrp1MO1 had been injected, but incomplete loss of detectable expression possibly due to diffusion of secreted sfrp1 protein from the uninjected right side of the embryo and cell mixing across the ventral midline. Image published in: Gibb N et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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