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Fig. S6. β-gal lineage tracing reveals cell fate changes within the embryo. (A) sfrp1MO1 was injected into both dorsal blastomeres at the 4-cell stage followed by 400 pg β-gal-encoding mRNA into one D21 cell at the 16-cell stage. (B,C) X-gal staining in whole embryos at stage 32 showing the lineage patterning of injected β-gal. (D,E) Whole-mount RNA in situ hybridisation for tnni3 expression on β-gal-injected embryos. CoMO (D) and sfrp1MO1 (E) injected embryos were sectioned at 14 μm. tnni3 expression was located at the myocardium (arrowhead) with co-expression of β-gal on the injected side. Note reduced tnni3 expression domain in E compared with D but equal β-gal expression. Scale bars: 100 μm. Image published in: Gibb N et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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