Xenbase Image ID: 80972

Fig. S2. Germ layer specification and anterior-posterior patterning are unaffected by knockdown of Osr1 and Osr2 on 1 side of the embryo. (A-D) Embryos were injected at the four/eight-cell stage on 1 side with control MM-MO1+MM-MO2 (10 ng each) or Osr1-MO+Osr2-MO (10 ng each), cultured until (A) gastrula, (B) neurula, (C) tailbud (C) or (D) stage 35, and assayed by in situ hybridization with the indicated probes. The earliest defect observed in Osr1/2-MO embryos was a loss of lim1, pax8 and raldh2 (red arrows) in the intermediate mesoderm of the developing kidney, as previously described (Tena et al., 2007). Liver, pancreas, intestine and heart development are unaffected in Osr1/2 morphants (D). pdx1 expression in the duodenum is modestly reduced by Osr1/2-MOs. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xtr Wt + osr1 MO + osr2 MO + NF18-20 (in situ hybridization) fig.S2.b Expression Phenotype decreased amount aldh1a2 expression in intermediate mesoderm decreased amount lhx1 expression in intermediate mesoderm Xtr Wt + osr1 MO + osr2 MO + NF23-24 (in situ hybridization) fig.S2.c Expression Phenotype decreased amount aldh1a2 expression in intermediate mesoderm decreased amount pax8 expression in pronephric mesenchyme Larger Image Printer Friendly View

Return to previous page