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Fig. S3. The Osr1/2-MO phenotype can be rescued by overexpression of mouse Osr1 RNA. Embryos were co-injected with control MM-MOs or Osr1/2-MOs and GR-mOsr1RNA (250 pg) in both dorsal-vegetal blastomeres at the eight-cell stage (bilateral injection) and then cultured with or without dexamethasone from stage 20 onwards. Analysis by in situ hybridization of nkx2.1, wnt2b, sox2 and bmp4 at stage 34/35 and of sftpc in isolated gut tubes at stage 42. Analysis by immunofluorescence of fibronectin (red), activated caspase 3 (green) and nuclei (blue) at stage 42 show and undivided foregut tube and extensive cell death. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.S3 Expression Phenotype mislocalised bmp4 expression in lateral plate mesoderm decreased amount nkx2-1 expression in lung bud decreased amount sox2 expression in foregut decreased amount wnt2b expression in lung bud Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (immunohistochemistry) fig.S3 Anatomical Phenotype abnormal development of esophagus abnormal development of trachea increased apoptosis in embryo Xtr Wt + Mmu.osr1-GR + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.S3 Expression Phenotype decreased amount sftpc expression in foregut Larger Image Printer Friendly View

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