Xenbase Image ID: 80976

Fig. S6. Retinoic acid signaling is required for Xenopus lung bud growth but not specification. (A,B) Embryos were cultured in 10 µM BMS493, a pan retinoic acid receptor inhibitor, from stages 25 to 35 (A) or stage 25-42 (B), and assayed by in situ hybridization with the indicated probes. (A) Although inhibition of RA signaling did not prevent pulmonary specification (Fig. 3), expression of dkk1 was upregulated as expected based on the finding that Dkk1 is upregulated in Raldh2-/- mutant mouse embryos (Chen et al., 2010). (B) A dorsal view of isolated gut tubes shows that inhibition of RA signaling from stages 25-42 inhibited the growth of sftpc-expressing lung buds. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue dkk1.L dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 35 and 36 brain spinal cord eye lens otic vesicle pharynx pharyngeal region stomodeal-hypophyseal primordium pronephric kidney midbrain-hindbrain boundary sftpc.L SP-C, SPC, surfc, xSP-C X. laevis Throughout NF stage 42 respiratory system lung Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + BMS493 + NF35/36 (in situ hybridization) fig.S6.a Expression Phenotype increased amount dkk1 expression in brain increased amount dkk1 expression in eye increased amount dkk1 expression in intermediate mesoderm increased amount dkk1 expression in pharyngeal region increased amount dkk1 expression in spinal cord increased amount dkk1 expression in ventral trunk Xtr Wt + BMS493 + NF35/36 (in situ hybridization) fig.S6.b Expression Phenotype decreased amount sftpc expression in lung bud Xtr Wt + BMS493 + NF42 (in situ hybridization) fig.S6.b Anatomical Phenotype decreased size of the lung bud Larger Image Printer Friendly View

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