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XB-IMG-80978

Xenbase Image ID: 80978


Fig. S8. BMP signaling that function in a time period after respiratory specification is required for trachea formation and lung bud development. (A,B) In situ hybridization of nkx2.1 expression in embryos treated from stages 35-42 with DMSO (vehicle) or 10 µM LDN193189 to inhibit BMP receptor type I signalling. Red arrows show absent trachea and single dismorphic lung bud. Brain, br; trachea, tr; lung bud, lb. (C,D) Immunofluorescence analysis of Nkx2.1 (green) and Sox2 (red) in serial foregut sections (1 is the anterior-most section, 4 is the posterior-most) reveal loss of trachea, expansion of esophagus and lung bud hypoplasia in LDN-treated embryos.

Image published in: Rankin SA et al. (2012)

Copyright © 2012. Image reproduced with permission of the Publisher.

Experiment + Assay Source Phenotypes and Disease
Xtr Wt + LDN-193189 + NF42 (in situ hybridization) fig.S8.b
Anatomical Phenotype
abnormal agenesis of trachea
decreased size of the lung bud
Expression Phenotype
decreased amount nkx2-1 expression in lung bud
decreased amount nkx2-1 expression in trachea
Xtr Wt + LDN-193189 + NF42 (immunohistochemistry) fig.S8.d1,d2,d3
Anatomical Phenotype
abnormal agenesis of trachea
abnormal esophagus morphology
Expression Phenotype
decreased amount nkx2-1 expression in trachea
Xtr Wt + LDN-193189 + NF42 (immunohistochemistry) fig.S8.d3,d4
Anatomical Phenotype
decreased size of the lung bud
Expression Phenotype
decreased amount nkx2-1 expression in lung bud

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