Xenbase Image ID: 80998

Fig. 3. Tcf21 associates with co-repressor proteins and is phosphorylated at multiple sites. (A) Experimental workflow for Tcf21 immunoaffinity purification (IP), and identification of Tcf21 interactions and post-translational modifications (PTMs). (B) Identification of Tcf21 peptide by CID MS/MS analysis. #916;M, ppmrefers to the difference between experimental and theoretical peptide masses in parts per million. (C) IP validation of interaction between Tcf21 and Ctbp2 in HEK293 cells. Both proteins run at ~50 kDa. WB, western blot. (D) Expression of Tcf21 protein interaction candidates, as identified by mass spectrometry, in Xenopus stage 45 cardiac tissue analyzed by RNA-seq read counts. Huwe1 and Tcf12 were not included in the RefSeq library used for alignment. (E) Enriched putative Tcf21 interactions, color-coded according to their GO annotations for biological processes. (F) Map of identified Tcf21 phosphorylation sites on serine residues. Image published in: Tandon P et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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