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Fig. 5. Tcf21 functions to promote epicardial maturation and epithelialization. Transverse sections (dorsal to the top) through the cardiac region of stage 46 CA:GFP transgenic embryos showing myocardium (green), cell nuclei stained with DAPI (blue) and immunohistochemical stains for (A-B″) aPKC, (C-D″) vimentin and (E-F″) laminin in red. Arrowheads highlight epicardial cells with increased or mislocalized staining compared with controls. Boxes indicates the regions magnified in A′-F′ and A″-F″. a, atrium; oft, outflow tract; ven, ventricle. Scale bars: 50 μm in A,C,E; 10 μm in A′,C′,E′; 5 μm in C″; 2.5 μm in A″,E″. Image published in: Tandon P et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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