Xenbase Image ID: 81001

Fig. 6. Epicardial cell migration is regulated by Tcf21. (A,B) Brightfield images of control (A) and Tcf21-depleted (B) cardiac explant on collagen gel. The dashed line indicates the extent of epicardial outgrowth after 16 hours of culture. (C,D) Imaris tracking software depicts individual epicardial cell movements during culture, comparing control (C) and Tcf21-depleted (D) epicardial behavior. (E,F) A significantly increased rate of epicardial cell migration (E) and total cell outgrowth (F) in a 48-hour culture period was observed in Tcf21-depleted as compared with control embryos. ****P<0.0001, two-tailed unpaired non-parametric Mann-Whitney test. Mean s.d. from a total of three independent experiments with the number of (E) migrating cells from 27 control and 34 Tcf21-depleted explants or (F) hearts assayed indicated in parentheses. epic, epicardial cells; oft, outflow tract; ven, ventricle. Scale bar: 200 μm. Image published in: Tandon P et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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