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Fig. S2. Analysis of Wt1-depleted embryos. (A-H) Brightfield images of live embryos at (A,B) stage 28, (C,D) stage 40, (E,F) stage 43, (G,H) stage 46. (I) Quantification of pericardial edema observed in embryos at stages 44-46; total embryos in brackets, two independent experiments, P<0.0001 by two-tailed Fisher exact test. Likelihood ratio c2 N=362, DF=1=259.856; P<0.0001, Pearson c2 N=362, DF=1=234.094; P<0.0001. (J-M) SEM images of stage 45 hearts depicting normal epicardial sheet migration over the heart in both (J,L) control and (K,M) Wt1-depleted embryos. Boxed areas indicate the regions of the ventricular surface magnified in L,M. Scale bar: 10 μm.

Image published in: Tandon P et al. (2013)

Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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