XB-IMG-81020
Xenbase Image ID: 81020
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Fig. 3. Xenopus Osr morphant embryos have severely impaired kidneys. (A–H) Lateral views of stage 25 Xenopus tropicalis embryos injected with 20 ng of MOXOsr1 (A–D) or 20 ng of MOXOsr2 (E–H) and 300 pg of LacZ mRNA to determine the injected side. Purple staining shows the expression of Xlim1 (A, B, E, F) or XPax8 (C, D, G, H), and brown staining the somitic muscles, labeled with the monoclonal antibody 12/101. The MO injected embryos show a reduced expression of the kidney markers on the injected sides (arrows in panels B, D, F and H; compare with the control sides shown in panels A, C, E and G). (I, M) Transverse section of stage 25 MOXOsr1 (I) or MOXOsr2 (M) injected embryos triple labeled for Xlim1 (pronephros, purple), muscles (brown) and Sox2 (neural tissue, cyan). Note the strong reduction of the pronephric tissue in the injected sides (arrows). (J–L and N–P) Stage 37 Xenopus tropicalis embryos injected with MOXOsr1 (J–L) or MOXOsr2 (N–P) and stained with the monoclonal antibody 3G8. Note the strong reduction of the kidney tissue in the injected sides (arrows in panels K, L, O, P). Insets are closer views. This reduction is clearly visible in transverse sections (arrow in panels L and P). (Q–T) Lateral views of stage 35 Xenopus tropicalis embryos co-injected with MOXOsr1 (Q, R) or MOXOsr2 (S–T) and Dextran-Fluorescein in the V2.2 blastomere at the 8–16 cell stage. The expression of XSGLT1K (Q, S; purple) and XNKCC2 (R, T; purple) is impaired in the injected side (Fluorescein distribution is visible in cyan). Brown staining in panels Q and T shows the somitic muscles labeled with the monoclonal antibody 12/101. Insets show the control un-injected side. (U) Target sequences for Xenopus Osr Morpholinos (MOs). In all sequences, the first methionine of the corresponding gene is underlined. Identical bases are in blue and mismatches in red. Note that the MOs for each Xenopus Osr gene have one mismatch with the corresponding Xenopus laevis and Xenopus tropicalis target sequences. In contrast, the MO against one of the paralogues has five or more mismatches with the sequence of the other gene. MOs with only one mismatch can efficiently block translation while five or more mismatches make an MO inactive. Image published in: Tena JJ et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |