XB-IMG-81088
Xenbase Image ID: 81088
|
||||||||||||||||||||
|
Fig. 3. Myocardin activates ectopic
expression of myocardial markers
in the Xenopus embryo. (A-H) 125
pg of myocardin mRNA was
injected into one cell of an eightcell
embryo, which was then
assayed for cardiac markers by
whole-mount in situ hybridization.
No expression of the MHCα gene
is observed in uninjected stage 14
embryos (A), however widespread
transcription of MHCα is observed
in myocardin-injected embryos
(B). Similarly, cardiac α-actin is
observed specifically in the presomitic
mesoderm at stage 14
control embryos (C), while
myocardin injected embryos
display widespread expression of
cardiac α-actin on the side of
injection (D). (E) Section through
the embryo in D shows ectopic
cardiac α-actin expression
(arrows) in the ectodermal and
mesodermal tissue layers. Ectopic
cardiac marker expression is not
observed in endodermal tissues.
(F) MHCα expression is heartspecific
at stage 28 in un-injected control embryos, but myocardin overexpression, (G), causes MHCα transcription in ectopic locations. Arrows
indicate normal cardiac expression. (H) Section through the embryo in G shows patches of ectopic MHCα expression in the neural tube (nt) and
eye. (I) Fluorescence microscopy of a stage 29 Xenopus embryo co-transgenic for NβT-GFP and NβT-myocardin showing GFP expression in
neural tissues. (J) In situ hybridization analysis of NβT-GFP/NβT-myocardin co-transgenic embryos using a MHCα probe shows ectopic
expression of MHCα in neural tissues. Image published in: Small EM et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published Larger Image Printer Friendly View |