XB-IMG-81095
Xenbase Image ID: 81095
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FIG. 2. First and last photo of photorecord of case SI 2631,
prepared by orthotopic transplantation of dorsal (1 o'clock) germinal
cells from the right eye of a stage-34 pigmented (XBL hybrid) donor
embryo into the right eye of a stage-34 albino host embryo;
magnification xl. (a) In a lateral view of the transilluminated
stage-40 embryo, the transplant had blackened and was seen with
reference to the eye and head, including the opaque central circle that
is the site for the yet undeveloped pupil and lens and to the ventral
fissure (vf). (b-d) Three views, at the same magnification as in a, of
the chimeric eye after 131 days of larval growth to stage-63/4. (b) In
lateral view, pigmented cells on the front of the eye could be assigned
to the iris (ir), whose projection on the eye surface occupies the halo
territory immediately encircling the pupil (p); the GZ (gz), which
occupies the halo domain just proximal to the ir on the rim of the
PRE; and the PRE that extends smoothly from the gz round the back
of the eye. (c) The dorsal view is dominated by the PRE (pre), but
also shows the lens and cornea protruding laterally. The spatiotemporal
axis of cell birthdates in the pre (as inferred from the data
in Fig. 3) extends from the back of the eye round to the pre margin
and more recently added cell groups are marked by smaller arrows.
(d) An unobstructed medial view of the eye-photographed after
enucleation, fixation, and removal of the lens and sclera-allowed
the oldest transplant cells to be seen on the back of the eye, with
reference to the optic nerve (on) and ventral fissure (vf). Image published in: Hunt RK et al. (1987) Copyright © 1987. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |