Xenbase Image ID: 81293

Figure 2. Sizzled Is Required for Foregut Organogenesis(A and B) Lineage labeling by a fluorescent dextran (FLDX) coinjected with a szl-MO (20 ng) into the C1/D1 cells of the 16-cell-stage embryo targets the dorsal-anterior mesendoderm at gastrula stage 10.5 (A), which gives rise to the ventral foregut progenitors at stage 20 (B).(C and D) A comparison to szl expression shows that these injections avoid the szl-expressing ventral mesoderm at stage 10.5, but targets foregut szl at stage 20. d, dorsal; v, ventral; a, anterior; p, posterior.(E) In situ hybridization of control and szl-MO-injected embryos demonstrates that Szl is required for foregut organogenesis. At stage 20, szl-MO embryos failed to maintain hhex expression, while nxk2.5 in the precardiac mesoderm was unaffected (E). Analysis of pdx1 (pancreas), nkx2.1 (lung/thyroid), hhex (liver/thyroid), and tnlc (heart) at stage 35 demonstrates that Szl is required for foregut organ specification.(Q and R) H&E staining of stage 37 embryos reveals a reduction in foregut size (dashed outline) and cell sloughed into the pharynx of szl-MO.(S and T) Isolated gut tubes at stage 42 show that szl-MO lack foregut organ buds (lu, lung; lv, liver; p, pancreas).See also Figure S2. Image published in: Kenny AP et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + bmp2 MO + NF20 (in situ hybridization) fig.2.f Expression Phenotype decreased amount hhex.L expression in foregut endoderm Xla Wt + bmp2 MO + NF37/38 (in situ hybridization) fig.2.j Expression Phenotype decreased amount pdx1.L expression in hepato-pancreatic progenitor cell Xla Wt + bmp2 MO + NF37/38 (in situ hybridization) fig.2.l Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the head Expression Phenotype decreased amount nkx2-1.L expression in lung primordium Xla Wt + bmp2 MO + NF37/38 (in situ hybridization) fig.2.n Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the head Expression Phenotype decreased amount hhex.L expression in liver decreased amount hhex.L expression in thyroid primordium Xla Wt + bmp2 MO + NF37/38 (in situ hybridization) fig.2.p Expression Phenotype decreased amount tnni3.L expression in heart Xla Wt + bmp2 MO + NF37/38 (morphology) fig.2.r Anatomical Phenotype decreased size of the foregut Xla Wt + bmp2 MO + NF42 (morphology) fig.2.t Anatomical Phenotype absent foregut absent liver primordium absent lung primordium absent ventral pancreatic bud Larger Image Printer Friendly View

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