XB-IMG-81306
Xenbase Image ID: 81306
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Figure S2. Targeted injection of the szl-MO into the foregut does not disrupt gastrula axial pattering. Embryos were either (A) uninjected or injection with the szl-MO (20 ng) into (B) C1/D1 cells at the 16-cell stage, which give rise to the foregut or (C) injected into ventral cells at the 4-cell stage. Injection of szl-MO into the C1/D1 presumptive foregut (B) did not alter gastrula stage axial pattering as indicated by no change in hhex or szl expression in the organizer or ventral mesoderm respectively, and no alterations in pSmad1/5/8 immunostaining. In contrast injection of the szl-MO into the ventral cells of the 4-cell- stage embryo resulted in axial pattering defects with elevated pSmad1/5/8, expanded expression of szl (a bmp-target) and a loss of hhex, all of which are consistent with previously published work showing that Szl in the gastrula mesoderm regulates axial pattering by repressing BMP signaling. Thus injection of the szl-MO into different regions of the embryos reveals distinct temporal and spatial Szl functions. Image published in: Kenny AP et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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