Xenbase Image ID: 81311

Figure S6. Characterization of fn-MO injected embryos. Embryos were injected with either control-MO or fibronectin-MOs (a combination of fn1- MO and fn2-MO; 15 ng each), which target both fn genes in the C1/D1 cells of the 16- cell stage embryo, which targets the anterior mesendoderm. FN immunostaining (80 m Z-projection) of the foregut at stage 20 shows that the pericellular FN matrix as well as the FN fibril layer between the mesoderm (mes) and endoderm (end) (yellow arrows) is absent in fn-MO embryos (red arrows), whereas the FN layer between the mesoderm and ectoderm (ect) is still present. hhex expression in the foregut is reduced at stage (st) 20 in fn-MO, but in the gastrula (Stage 10.5) hhex expression is unaltered. At stage 35 fn-MO exhibit reduced the liver marker for1 and cardia bifida as shown by the heart marker c-troponin. Controls injecting the fibronectin-MOs into all cells of the 4-cell stage embryo resulted in frequent gastrulation defects. Image published in: Kenny AP et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + fn1 MO + NF20 (in situ hybridization) fig.S6 Expression Phenotype decreased amount hhex.L expression in foregut Xla Wt + fn1 MO + NF35/36 (in situ hybridization) fig.S6 Anatomical Phenotype abnormal incomplete closing of the neural tube abnormally split primary heart field Expression Phenotype decreased amount nr1h5.L expression in liver primordium Larger Image Printer Friendly View

Return to previous page