Xenbase Image ID: 81341

Fig 3. Different regions of the embryo responded differently to changes in retinoic acid (RA) signaling. Addition of RA to embryos resulted in a general increase in staining by in situ hybridization, suggesting that the most cells are responding to the increased signaling. There was a much greater response at specific sites. In particular, there is a gap in staining in the neural tube (black arrowheads) roughly corresponding to the hindbrain. Addition of RA resulted in that region showing very strong expression of ski as assayed by in situ hybridization. The posterior spinal cord (blue arrow) does not normally have strong expression of ski but does so when RA is added. When embryos are treated with an RA antagonist, the strong staining in the brain (red arrow) and spinal cord (yellow arrows) is much reduced. Note that staining in the somites is also markedly reduced in the antagonist treated embryos. DMSO, dimethyl sulfoxide. Image published in: Melling MA et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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